The Adenosine Triphosphate Assay: Conceptions and Misconceptions

LH Stevenson; TH Chrzanowski; CW Erkenbrecher

doi:10.1520/STP36008S

SYMPOSIA PAPER Published: 01 January 1979

STP36008S

The Adenosine Triphosphate Assay: Conceptions and Misconceptions

Source

The present state of technology associated with the extraction and quantification of adenosine triphosphate (ATP) from aquatic samples is considered. A brief examination of the history of the method is presented, and data available from studies designed to determine the level of ATP in some aquatic environments are presented in a comparative manner. The possible interference of guanosine triphosphate in the assay procedure as well as irregularities produced by altering the luciferin concentration employed in the assay are described. Procedures currently employed for the extraction and measurement of ATP as an indication of microbial biomass are given.

Author Information

Stevenson, LH

University of South Carolina, and Belle W. Baruch Institute for Marine Biology and Coastal Research, University of South Carolina, Columbia, S.C.

Chrzanowski, TH

University of South Carolina, Columbia, S.C.

Erkenbrecher, CW

Old Dominion University, Norfolk, Va.

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Details

Developed by Committee: D19

Pages: 99–116

DOI: 10.1520/STP36008S

ISBN-EB: 978-0-8031-4760-7

ISBN-13: 978-0-8031-0526-3

The Adenosine Triphosphate Assay: Conceptions and Misconceptions

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