Measurement of Bacterial Biomass as Lipopolysaccharide

SW Watson; JE Hobbie

doi:10.1520/STP36006S

SYMPOSIA PAPER Published: 01 January 1979

STP36006S

Measurement of Bacterial Biomass as Lipopolysaccharide

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The biomass of bacteria in various waters can be estimated by measuring the amount of cellular bound lipopolysaccharide (LPS). LPS, which is present in the cell walls of gram-negative bacteria, including cyanobacteria, can be quantitated by using an extract of Limulus amebocytes. In one special environment, an oceanic water column below the euphotic zone, the results of the LPS technique agreed very well with those of the direct count and adenosine triphosphate techniques for measuring bacterial biomass.

Author Information

Watson, SW

Woods Hole Oceanographic Institution, Woods Hole, Mass.

Hobbie, JE

The Ecosystems Center, Marine Biological Laboratory, Woods Hole, Mass.

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Details

Developed by Committee: D19

Pages: 82–88

DOI: 10.1520/STP36006S

ISBN-EB: 978-0-8031-4760-7

ISBN-13: 978-0-8031-0526-3

Measurement of Bacterial Biomass as Lipopolysaccharide

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