SYMPOSIA PAPER
Published:
01 June 1979
STP34967S
Seed Impregnation with Rhodamine B Dye for Studying Wild Animals
SourceIn recent studies on protective measures for direct seeding of conifers, we developed methods for tracing the consumption of chemically treated and untreated Douglas-fir (Pseudotsuga menziesii) seeds by deer mice (Peromyscus maniculatus), dark-eyed juncos (Junco hyemalis), and other seed-eating animals in the Pacific Northwest from the time of sowing of seed in the fall until germination in the spring (about five months). We impregnated the seeds with rhodamine B and identified the dye in feces from live and dead animals by alcohol extraction and thin-layer chromatography. The rhodamine B-impregnation, recovery, and identification methods appear promising for use in other studies.
Author Information
Lindsey, GD
U.S. Fish and Wildlife Service, Forestry Sciences Laboratory, Olympia, Wash
Heebner, CF
Anthony, RM
U.S. Fish and Wildlife Service, Silviculture Laboratory, Bend, Ore
Evans, J.
U.S. Fish and Wildlife Service, Forestry Sciences Laboratory, Olympia, Wash
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Details
Developed by Committee: E35
Pages: 118–122
DOI: 10.1520/STP34967S
ISBN-EB: 978-0-8031-4748-5
ISBN-13: 978-0-8031-0594-2