A Radiorespirometric Study of the Effects of a Chemical Substance on Carbohydrate Metabolism in the Intact Fish

DHW Liu

doi:10.1520/STP34174S

SYMPOSIA PAPER Published: 01 January 1981

STP34174S

A Radiorespirometric Study of the Effects of a Chemical Substance on Carbohydrate Metabolism in the Intact Fish

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The effects of potassium pentachlorophenate (KPCP) on carbohydrate metabolism in the fish (Cichlasoma bimaculatum) were studied using radiorespirometry. Analysis of the patterns of radiolabeled carbon dioxide (¹⁴CO₂) recovery from fish exposed to 0, 0.2, and 1.5 ppm of KPCP and metabolizing specifically labeled carbon-14 (¹⁴C)-glucose and acetate indicated that this species of fish uses the Embden-Meyerhoff-Parnas (EMP), pentose phosphate (PP), and glucuronic acid (GA) pathways in conjunction with the tricarboxylic acid (TCA) cycle for metabolizing carbohydrates. The KPCP stimulated the EMP pathway and the TCA cycle and presumably reduced the utilization of the PP and GA pathways.

Author Information

Liu, DHW

Woodward-Clyde Consultants, San Francisco, Calif.

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Details

Developed by Committee: D19

Pages: 449–458

DOI: 10.1520/STP34174S

ISBN-EB: 978-0-8031-4803-1

ISBN-13: 978-0-8031-0799-1

A Radiorespirometric Study of the Effects of a Chemical Substance on Carbohydrate Metabolism in the Intact Fish

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