Metabolites of Benzo[ ]pyrene in Aroclor 1254-Treated Mullet

B Tan; MV Kilgore; DL Elam; P Melius; WP Schoor

doi:10.1520/STP34160S

SYMPOSIA PAPER Published: 01 January 1981

STP34160S

Metabolites of Benzo[ ]pyrene in Aroclor 1254-Treated Mullet

Source

This paper presents a systematic approach to the separation and characterization of benzo[a]pyrene (BaP) metabolites using the efficient analytical technique of high-pressure liquid chromatography (HPLC). Hepatic supernatant (S-9) preparations from Aroclor 1254-treated mullet (Mugil cephalus) were used to study the in vitro metabolism of BaP. The lipid-soluble hydroxylated products were extracted in ethyl acetate prior to HPLC analysis. Three diols (9,10; 7,8; and 4,5) and two phenols (9 and 3) of BaP were isolated and identified. From their relative retention times, the other metabolites noted were likely to be unresolvable quinones. The results obtained in the mullet were compared with those obtained in the rat (Rattus norvegicus).

Author Information

Tan, B

Auburn University, Auburn, Ala.

Kilgore, MV

Auburn University, Auburn, Ala.

Elam, DL

Auburn University, Auburn, Ala.

Melius, P

Auburn University, Auburn, Ala.

Schoor, WP

U.S. Environmental Protection Agency, Environmental Research Laboratory, Sabin Island, Gulf Breeze, Fla.

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Details

Developed by Committee: D19

Pages: 239–246

DOI: 10.1520/STP34160S

ISBN-EB: 978-0-8031-4803-1

ISBN-13: 978-0-8031-0799-1

Metabolites of Benzo[ ]pyrene in Aroclor 1254-Treated Mullet

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