Analysis of Heterogeneous Emissions by Multifrequency Phase and Modulation Fluorometry

DM Jameson; E Gratton

doi:10.1520/STP31868S

SYMPOSIA PAPER Published: 01 January 1983

STP31868S

Analysis of Heterogeneous Emissions by Multifrequency Phase and Modulation Fluorometry

Source

The technique of multifrequency phase and modulation fluorometry may be applied to the measurement and analysis of heterogeneous fluorescence lifetimes. This paper addresses two approaches to the data analysis: (1) the exact analytical solution (Weber's algorithm) for determining the lifetimes of N components and their relative contributions using N modulation frequencies and (2) a least-squares method. The mathematical rationale for each approach is described and the effects of random or systematic errors on the results obtained with each method are discussed. The relative merits of each approach are considered in light of results on both real and simulated data.

Author Information

Jameson, DM

University of Texas, Health Science Center at Dallas, Dallas, TX

Gratton, E

University of Illinois at Urbana-Champaign, Urbana, IL

Shipping & Handling

Domestic Shipping

Domestic orders are delivered via United Parcel Service (UPS) or United States Postal Service (USPS). Transit times average 3 to 5 business days. Please be aware that UPS will not deliver packages to Post Office Boxes.

Domestic Rush Orders

Please Contact Us.

International Standard Shipping

International orders are delivered via courier post services which can be either a postal service, courier service, or a combination of both. Standard Service is untraceable. Please allow 4-7 weeks for delivery.

International Expedited Shipping

Please Contact Us.

Please be aware that carriers will not deliver packages to Post Office Boxes. Because of the variability of customs processes and procedures in different countries, ASTM International cannot guarantee transit times to international destinations. Customs duty and taxes are the responsibility of the consignee.

Shipping & Handling charges follow the rate schedule, below:

Order Total	Shipping & Handling Fee (US Domestic)
Up to $50.00	$18.72
$50.01 to $100.00	$20.80
$100.01 to $150.00	$29.52
$150.01 to $250.00	$39.09
$250.01 to $500.00	$56.25
$500.01 to $750.00	$76.42
$750.01 to $1000.00	$93.15
$1000.01 to $1500.00	$121.27
$1500.01 to $2500.00	$158.38
$2500.01 to $4999.00	$209.04
$5000.00 to higher	FREE

Order Total	Shipping & Handling Fee (International)
Up to $50.00	$68.72
$50.01 to $100.00	$70.80
$100.01 to $150.00	$79.52
$150.01 to $250.00	$89.09
$250.01 to $500.00	$106.25
$500.01 to $750.00	$126.42
$750.01 to $1000.00	$143.15
$1000.01 to $1500.00	$171.27
$1500.01 to $2500.00	$208.38
$2500.01 to $4999.00	$259.04
$5000.00 to higher	FREE

Shipping and Handling charges are approximate. Additional charges may be incurred if your order requires multiple shipments. This does not apply to complete sets and sections.

Reprints and Permissions

Reprints and copyright permissions can be requested through the
Copyright Clearance Center

Details

Developed by Committee: E13

Pages: 67–81

DOI: 10.1520/STP31868S

ISBN-EB: 978-0-8031-4887-1

ISBN-13: 978-0-8031-0212-5

Analysis of Heterogeneous Emissions by Multifrequency Phase and Modulation Fluorometry

Author Information

Related

Reprints and Permissions

Details