Application of Computerized Quantitative Infrared Spectroscopy to the Determination of the Principal Lipids Found in Blood Serum

GL McClure; PB Roush; JF Williams; CA Lehmann

doi:10.1520/STP23166S

SYMPOSIA PAPER Published: 01 January 1987

STP23166S

Application of Computerized Quantitative Infrared Spectroscopy to the Determination of the Principal Lipids Found in Blood Serum

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This paper discusses the issues related to the development of a practical infrared spectroscopic method for the determination of the principal types of serum lipids, triglycerides, cholesteryl esters, and phospholipids. Earlier papers on this subject are noted, and the various matrix mathematical approaches used previously for this application are compared. The results described in this work were obtained with a least squares curve fitting type of algorithm, termed a Q-matrix approach. Comparisons are made between results obtained with a number of different methods constructed for use with the Q-matrix approach.

Author Information

McClure, GL

Perkin-Elmer, Corp, Norwalk, CT

Roush, PB

Perkin-Elmer, Corp, Norwalk, CT

Williams, JF

Perkin-Elmer, Corp, Norwalk, CT

Lehmann, CA

School of Applied Health Professions, State University of New York, Stony Brook, NY

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Details

Developed by Committee: E13

Pages: 131–154

DOI: 10.1520/STP23166S

ISBN-EB: 978-0-8031-5001-0

ISBN-13: 978-0-8031-0929-2

Application of Computerized Quantitative Infrared Spectroscopy to the Determination of the Principal Lipids Found in Blood Serum

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