Identification of Aneuploidy-Inducing Agents Using the Wheat Seedling Assay

SS Sandhu; JS Dhesi; BS Gill

doi:10.1520/STP19527S

SYMPOSIA PAPER Published: 01 January 1991

STP19527S

Identification of Aneuploidy-Inducing Agents Using the Wheat Seedling Assay

Source

A short-term assay using Chinese Spring wheat for detecting induced aneuploidy and/or clastogenicity is described. Seeds of virescent wheat (v₁v₁) are treated with a test agent. The appearance of white sectors (v₁v₁v₁) or green sectors (v₁0) on the virescent leaves indicates the gain or loss of a chromosome or a segment of a chromosome-bearing v₁ allele. Three test chemicals, chromium trioxide (100 to 5000 (μg/mL), chromium dichromate (500 to 5000 μg/mL), and cyclophosphamide (100 to 1000 μg/mL) produced a dose-related positive response for chromosome aberrations in this assay.

Author Information

Sandhu, SS

U.S. Environmental Protection Agency, Research Triangle Park, NC

Dhesi, JS

North Carolina Central University, Durham, NC

Gill, BS

Environmental Health Research and Testing, Inc., Research Triangle Park, NC

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Details

Developed by Committee: E47

Pages: 326–330

DOI: 10.1520/STP19527S

ISBN-EB: 978-0-8031-5173-4

ISBN-13: 978-0-8031-1422-7

Identification of Aneuploidy-Inducing Agents Using the Wheat Seedling Assay

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