Towards Monitoring of Cell Growth in Tissue Engineering Scaffolds: High Resolution Optical Techniques

MT Cicerone; JP Dunkers; NR Washburn

doi:10.1520/STP11635S

SYMPOSIA PAPER Published: 01 January 2004

STP11635S

Towards Monitoring of Cell Growth in Tissue Engineering Scaffolds: High Resolution Optical Techniques

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Inability to obtain cellular-level information about progress of cell growth in tissue-engineering (TE) scaffolds is a pervasive problem. We demonstrate that a confocal microscope with two collinear contrast mechanisms, optical coherence, and fluorescence, can be used to perform non-destructive imaging of a polymer TE scaffold containing osteoblasts. We show that the combination of the techniques shows promise for in situ measurements of cell growth in a bioreactor, even for highly opaque TE scaffolds.

Author Information

Cicerone, MT

National Institute of Standards and Technology, Gaithersburg, MD

Dunkers, JP

National Institute of Standards and Technology, Gaithersburg, MD

Washburn, NR

National Institute of Standards and Technology, Gaithersburg, MD

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Developed by Committee: F04

Pages: 59–66

DOI: 10.1520/STP11635S

ISBN-EB: 978-0-8031-5497-1

ISBN-13: 978-0-8031-3471-3

Towards Monitoring of Cell Growth in Tissue Engineering Scaffolds: High Resolution Optical Techniques

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