Journal Published Online: 01 November 1990
Volume 35, Issue 6

Effects of Cytosine Methylation at Restriction Sites on Deoxyribonucleic Acid (DNA) Typing

CODEN: JFSCAS

Abstract

The effects of endogenous 5-methylcytosines on deoxyribonucleic acid (DNA) fingerprints were studied. Analysis with methylation-sensitive restriction endonuclease Sau3AI and its methylation-insensitive isoschizomer MboI showed some differences in the patterns generated as a result of 5-methylcytosines at the recognition sites. Moreover, a few bands of sperm DNA did not match those of blood DNA from the same individual, a phenomenon only observed in the digests of methylation-sensitive endonucleases. These findings indicate the unsuitability of methylation-sensitive restriction endonucleases for DNA fingerprinting and other forms of DNA typing, because of the tissue-specific status of the methylation.

Author Information

Washio, K
Faculty of Science, The University of Tokyo, Tokyo, Japan
Ueda, S
Faculty of Science, The University of Tokyo, Tokyo, Japan
Misawa, S
Institute of Community Medicine, University of Tsukuba, Ibaraki, Japan
Pages: 7
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Stock #: JFS12961J
ISSN: 0022-1198
DOI: 10.1520/JFS12961J