Standard
Historical
Last Updated: Jan 06, 2023
Track Document
ASTM E388-04(2015)
Standard Test Method for Wavelength Accuracy and Spectral Bandwidth of Fluorescence Spectrometers
Standard Test Method for Wavelength Accuracy and Spectral Bandwidth of Fluorescence Spectrometers
E0388-04R15
ASTM|E0388-04R15|en-US
Standard Test Method for Wavelength Accuracy and Spectral Bandwidth of Fluorescence Spectrometers
Standard
E388 Standard Test Method for Wavelength Accuracy and Spectral Bandwidth of Fluorescence Spectrometers>
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Abstract
This test method covers the testing of the spectral bandwidth and wavelength accuracy of fluorescence spectrometers that use a monochromator for emission wavelength selection and photomultiplier tube detection. The method can be applied to instruments that use multi-element detectors, such as diode arrays, but results must be interpreted carefully. Atomic lines between 250 nm and 1000 nm are used in the method. The difference between the apparent wavelength and the known wavelength for a series of atomic emission lines is used as a test for wavelength accuracy. The apparent width of some of these lines is used as a test for spectral bandwidth.
Scope
1.1 This test method covers the testing of the spectral bandwidth and wavelength accuracy of fluorescence spectrometers that use a monochromator for emission wavelength selection and photomultiplier tube detection. This test method can be applied to instruments that use multi-element detectors, such as diode arrays, but results must be interpreted carefully. This test method uses atomic lines between 250 nm and 1000 nm.
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.